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J Chromatogr A. 2000 Dec;903(1-2):93-8.

Separation of spiramycin components using high-speed counter-current chromatography.

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  • 1Aichi Prefectural Institute of Public Health, Nagoya, Japan.


High-speed counter-current chromatography was successfully applied to the separation of the main components of spiramycin. A 25-mg quantity of sample was separated using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:6:5:5), and the fractions were analyzed by high-performance liquid chromatography and fast atom bombardment mass spectrometry. The separation yielded 13.4, 0.7 and 1.7 mg of spiramycins I, II, and III with purities of 98.2, 92.3 and 97.4%, respectively.

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