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J Comput Biol. 2000;7(5):717-29.

DNA sequencing with transposons.

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  • 1Department of Statistics, University of California, Berkeley, 367 Evans Hall, #3860, Berkeley, CA 94720-3860, USA. scawley@stat.berkeley.edu


The use of transposons offers the possibility of a directed approach to DNA sequencing, where a target DNA up to about 6kb in length can be sequenced quickly and with minimal redundancy. Transposons are mobile DNA elements which can be inserted in a reasonably random fashion into the target DNA. An important part of this process is the location of the transposon insertions (known as mapping) and the selection of a sensible subset of transposons to use as priming sites for sequencing reactions. This paper presents a probabilistic method of scoring selected subsets of transposons and a graph-theoretic algorithm for selection of a subset of maximal score.

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