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FEBS Lett. 2000 Dec 22;487(1):13-6.

Genomic exploration of the hemiascomycetous yeasts: 2. Data generation and processing.

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  • 1GENOSCOPE, Centre National de Séquençage, 2 rue Gaston Crémieux, P.O. Box 91, Cedex, F-Evry, France. artigue@genoscope.cns.fr


The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length.

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