Abstract
The uptake into isolated HeLa nuclei of radioactive cytosol proteins and purified Escherichia coli ribosomal protein L7 is stimulated up to 4-fold by pancreatic deoxyribonuclease (DNase I). Similar effects are not observed with pancreatic ribonuclease A or phospholipase C. The results reported suggest that there is a general stimulatory effect of DNase on protein uptake by nuclei.
MeSH terms
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Bacterial Proteins / metabolism*
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism*
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Dactinomycin / pharmacology
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Deoxyribonucleases / pharmacology*
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Ethidium / pharmacology
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HeLa Cells / drug effects
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HeLa Cells / metabolism*
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Leucine / metabolism
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Neoplasm Proteins / metabolism*
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Pancreas / enzymology*
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Phospholipases / pharmacology
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Ribonucleases / pharmacology
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Ribosomes / metabolism
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Tritium
Substances
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Bacterial Proteins
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Neoplasm Proteins
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Tritium
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Dactinomycin
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Deoxyribonucleases
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Phospholipases
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Ribonucleases
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Ethidium
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Leucine