Abstract
Fructose transporter (GLUT-5) expression is low in mid-weaning rat small intestine, increases normally after weaning is completed, and can be precociously induced by premature consumption of a high-fructose (HF) diet. In this study, an in vivo perfusion model was used to determine the mechanisms regulating this substrate-induced reprogramming of GLUT-5 development. HF (100 mM) but not high-glucose (HG) perfusion increased GLUT-5 activity and mRNA abundance. In contrast, HF and HG perfusion had no effect on Na(+)-dependent glucose transporter (SGLT-1) expression but increased c-fos and c-jun expression. Intraperitoneal injection of actinomycin D before intestinal perfusion blocked the HF-induced increase in fructose uptake rate and GLUT-5 mRNA abundance. Actinomycin D also prevented the perfusion-induced increase in c-fos and c-jun mRNA abundance but did not affect glucose uptake rate and SGLT-1 mRNA abundance. Cycloheximide blocked the HF-induced increase in fructose uptake rate but not the increase in GLUT-5 mRNA abundance and had no effect on glucose uptake rate and SGLT-1 mRNA abundance. In neonatal rats, the substrate-induced reprogramming of intestinal fructose transport is likely to involve transcription and translation of the GLUT-5 gene.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cycloheximide / pharmacology
-
Dactinomycin / pharmacology
-
Dose-Response Relationship, Drug
-
Female
-
Fructose / pharmacokinetics
-
Gene Expression Regulation, Developmental / physiology*
-
Glucose / pharmacokinetics
-
Glucose Transporter Type 5
-
Intestinal Absorption / physiology*
-
Intestinal Mucosa / growth & development
-
Intestinal Mucosa / metabolism
-
Intestine, Small / growth & development*
-
Intestine, Small / metabolism*
-
Male
-
Membrane Glycoproteins / genetics
-
Membrane Glycoproteins / metabolism
-
Monosaccharide Transport Proteins / genetics*
-
Monosaccharide Transport Proteins / metabolism
-
Perfusion
-
Pregnancy
-
Protein Synthesis Inhibitors / pharmacology
-
Proto-Oncogene Proteins c-fos / genetics
-
RNA, Messenger / metabolism
-
Rats
-
Rats, Sprague-Dawley
-
Sodium / metabolism
-
Sodium-Glucose Transporter 1
-
Survival Rate
-
Transcription, Genetic / drug effects
-
Transcription, Genetic / physiology
Substances
-
Glucose Transporter Type 5
-
Membrane Glycoproteins
-
Monosaccharide Transport Proteins
-
Protein Synthesis Inhibitors
-
Proto-Oncogene Proteins c-fos
-
RNA, Messenger
-
Slc5a1 protein, rat
-
Sodium-Glucose Transporter 1
-
Dactinomycin
-
Fructose
-
Cycloheximide
-
Sodium
-
Glucose