FEBS Lett. 2000 Nov 10;484(3):291-8.

Nuclear import factors importin alpha and importin beta undergo mutually induced conformational changes upon association.

Cingolani G, Lashuel HA, Gerace L, Müller CW.

Source

Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Abstract

A heterodimer of importin alpha and importin beta accomplishes the nuclear import of proteins carrying classical nuclear localization signals (NLS). The interaction between the two import factors is mediated by the IBB domain of importin alpha and involves an extended recognition surface as shown by X-ray crystallography. Using a combination of biochemical and biophysical techniques we have investigated the formation of the importin beta:IBB domain complex in solution. Our data suggest that upon binding to the IBB domain, importin beta adopts a compact, proteolytically resistant conformation, while simultaneously the IBB domain folds into an alpha helix. We suggest a model to describe how these dual mutually induced conformational changes may orchestrate the nuclear import of NLS cargo in vivo.

PMID:: 11078895; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Structure of importin-beta bound to the IBB domain of importin-alpha. [Nature. 1999]
Structure of importin-beta bound to the IBB domain of importin-alpha.
Cingolani G, Petosa C, Weis K, Müller CW. Nature. 1999 May 20; 399(6733):221-9.
Biophysical characterization of interactions involving importin-alpha during nuclear import. [J Biol Chem. 2001]
Biophysical characterization of interactions involving importin-alpha during nuclear import.
Catimel B, Teh T, Fontes MR, Jennings IG, Jans DA, Howlett GJ, Nice EC, Kobe B. J Biol Chem. 2001 Sep 7; 276(36):34189-98. Epub 2001 Jul 11.
Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta. [Mol Cell. 2002]
Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta.
Cingolani G, Bednenko J, Gillespie MT, Gerace L. Mol Cell. 2002 Dec; 10(6):1345-53.
Review The importin β binding domain as a master regulator of nucleocytoplasmic transport. [Biochim Biophys Acta. 2011]
Review The importin β binding domain as a master regulator of nucleocytoplasmic transport.
Lott K, Cingolani G. Biochim Biophys Acta. 2011 Sep; 1813(9):1578-92. Epub 2010 Oct 26.
Review [Nuclear pore-targeting complex/importin family]. [Gan To Kagaku Ryoho. 1997]
Review [Nuclear pore-targeting complex/importin family].
Imamoto N. Gan To Kagaku Ryoho. 1997 Sep; 24(11):1401-6.

See reviews... See all...

Cited by 7 PubMed Central articles

The biological function of the Huntingtin protein and its relevance to Huntington's Disease pathology. [Curr Trends Neurol. 2011]
The biological function of the Huntingtin protein and its relevance to Huntington's Disease pathology.
Schulte J, Littleton JT. Curr Trends Neurol. 2011 Jan 1; 5:65-78.
The importin beta binding domain modulates the avidity of importin beta for the nuclear pore complex. [J Biol Chem. 2010]
The importin beta binding domain modulates the avidity of importin beta for the nuclear pore complex.
Lott K, Bhardwaj A, Mitrousis G, Pante N, Cingolani G. J Biol Chem. 2010 Apr 30; 285(18):13769-80. Epub 2010 Mar 1.
Review The design of Förster (fluorescence) resonance energy transfer (FRET)-based molecular sensors for Ran GTPase. [Methods. 2010]
Review The design of Förster (fluorescence) resonance energy transfer (FRET)-based molecular sensors for Ran GTPase.
Kaláb P, Soderholm J. Methods. 2010 Jun; 51(2):220-32. Epub 2010 Jan 22.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Nuclear import factors importin alpha and importin beta undergo mutually induced...
Nuclear import factors importin alpha and importin beta undergo mutually induced conformational changes upon association.
FEBS Lett. 2000 Nov 10 ;484(3):291-8.

PubMed
A novel compound heterozygous mutation in the RDH5 gene in a patient with fundus...
A novel compound heterozygous mutation in the RDH5 gene in a patient with fundus albipunctatus.
Am J Ophthalmol. 2000 Nov ;130(5):672-5.

PubMed
The use of contrast-enhanced magnetic resonance imaging to identify reversible m...
The use of contrast-enhanced magnetic resonance imaging to identify reversible myocardial dysfunction.
N Engl J Med. 2000 Nov 16 ;343(20):1445-53.

PubMed
Highly active antiretroviral therapy and incidence of cancer in human immunodefi...
Highly active antiretroviral therapy and incidence of cancer in human immunodeficiency virus-infected adults.
J Natl Cancer Inst. 2000 Nov 15 ;92(22):1823-30.

PubMed
Arginine transport through system y(+)L in cultured human fibroblasts: normal ph...
Arginine transport through system y(+)L in cultured human fibroblasts: normal phenotype of cells from LPI subjects.
Am J Physiol Cell Physiol. 2000 Dec ;279(6):C1829-37.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Nuclear import factors importin alpha and importin beta undergo mutually induced conformational changes upon association.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 7 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information