Neurotrophic function of conditioned medium from human amniotic epithelial cells

J Neurosci Res. 2000 Nov 15;62(4):585-90. doi: 10.1002/1097-4547(20001115)62:4<585::AID-JNR13>3.0.CO;2-U.

Abstract

Human amniotic epithelial cells (HAEC) may have pluripotent function because they are formed from the epiblast cells at the 8th day of fertilization. Previously, we reported that HAEC have the capacity to synthesize and release acetylcholine and catecholamine associated with the binding sites of catecholamine receptors. We show the neurotrophic function of a conditioned medium from HAEC using cultured cortical neurons of E18 rats. Extensive analyses with various techniques demonstrated that HAEC and immortalized HAEC synthesize and release brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth factor (NGF). Other neurotrophic factors were not detected in a cultured medium of HAEC by enzyme immunoassay. Various neurotrophic factors or growth factors did not show neurotrophic effects on E18 rat neuron except for EGF. Because EGF was not detected in the conditioned medium of HAEC, these data indicate an unidentified neurotrophic factor presently that is synthesized and released from HAEC. The amniotic membrane may have a significant role in supplying neurotrophic factors to the amniotic fluid as well as neurotransmitters, suggesting an important function to the early stages of neural development in the embryo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amniotic Fluid / cytology
  • Amniotic Fluid / metabolism*
  • Animals
  • Brain-Derived Neurotrophic Factor / genetics
  • Brain-Derived Neurotrophic Factor / metabolism
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Cells, Cultured / cytology
  • Cells, Cultured / drug effects
  • Cells, Cultured / metabolism
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / embryology
  • Cerebral Cortex / metabolism
  • Culture Media, Conditioned / metabolism
  • Culture Media, Conditioned / pharmacology*
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism*
  • Female
  • Fetus
  • Glial Fibrillary Acidic Protein / metabolism
  • Humans
  • Microtubule-Associated Proteins / metabolism
  • Nerve Growth Factor / genetics
  • Nerve Growth Factor / metabolism
  • Nerve Growth Factors / biosynthesis
  • Nerve Growth Factors / pharmacology*
  • Neurons / cytology
  • Neurons / drug effects*
  • Neurons / metabolism
  • Neuroprotective Agents / metabolism
  • Neuroprotective Agents / pharmacology*
  • Neurotrophin 3 / genetics
  • Neurotrophin 3 / metabolism
  • Pregnancy
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar

Substances

  • Brain-Derived Neurotrophic Factor
  • Culture Media, Conditioned
  • Glial Fibrillary Acidic Protein
  • Microtubule-Associated Proteins
  • Nerve Growth Factors
  • Neuroprotective Agents
  • Neurotrophin 3
  • RNA, Messenger
  • Nerve Growth Factor