Abstract
The SpoIIIE protein of Bacillus subtilis is required for chromosome segregation during spore formation. The COOH-terminal cytoplasmic part of SpoIIIE was shown to be a DNA-dependent adenosine triphosphatase (ATPase) capable of tracking along DNA in the presence of ATP, and the NH(2)-terminal part of the protein was found to mediate its localization to the division septum. Thus, during sporulation, SpoIIIE appears to act as a DNA pump that actively moves one of the replicated pair of chromosomes into the prespore. The presence of SpoIIIE homologs in a broad range of bacteria suggests that this mechanism for active transport of DNA may be widespread.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Adenosine Triphosphate / metabolism
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Amino Acid Motifs
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Bacillus subtilis / metabolism*
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Bacterial Proteins / chemistry
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism*
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Biological Transport, Active
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DNA Helicases*
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DNA Topoisomerases, Type I / metabolism
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DNA, Bacterial / metabolism*
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DNA, Superhelical / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Sigma Factor*
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Spores, Bacterial / metabolism
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Transcription Factors*
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA, Superhelical
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Recombinant Proteins
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Sigma Factor
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Transcription Factors
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spoIIR protein, Bacillus subtilis
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spore-specific proteins, Bacillus
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Adenosine Triphosphate
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Adenosine Triphosphatases
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DNA Helicases
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DNA Topoisomerases, Type I