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Nat Cell Biol. 2000 Nov;2(11):805-11.

cAMP-GEFII is a direct target of cAMP in regulated exocytosis.

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  • 1Department of Molecular Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.

Abstract

Although cAMP is well known to regulate exocytosis in many secretory cells, its direct target in the exocytotic machinery is not known. Here we show that cAMP-GEFII, a cAMP sensor, binds to Rim (Rab3-interacting molecule, Rab3 being a small G protein) and to a new isoform, Rim2, both of which are putative regulators of fusion of vesicles to the plasma membrane. We also show that cAMP-GEFII, through its interaction with Rim2, mediates cAMP-induced, Ca2+-dependent secretion that is not blocked by an inhibitor of cAMP-dependent protein kinase (PKA). Accordingly, cAMP-GEFII is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-independent exocytosis.

[PubMed - indexed for MEDLINE]
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