Send to:

Choose Destination
See comment in PubMed Commons below
Neuron. 2000 Sep;27(3):461-8.

Active stabilization of electrodes for intracellular recording in awake behaving animals.

Author information

  • 1Biological Computation Research Department, Lucent Technologies, New Jersey 07974, USA.


Intracellular recording is a powerful electrophysiology technique that has revealed much of what is known about the biophysical properties of neurons. However, neuronal properties are strongly affected by activity dependent and modulatory influences, making it essential, ultimately, to study these properties in behaving animals. Unfortunately, intracellular recording has only been widely applied in vitro, since cardiac and respiratory pulsations make intracellular recording difficult in vivo. In awake behaving animals, spontaneous movements make intracellular recording nearly impossible. Here I present a novel technique to dynamically stabilize the position of a recording electrode relative to the brain. Physiological signals that are predictive of brain motion at the recording site, such as the electrocardiogram (EKG), respiratory pressure, or cranial motion, are used to control a piezoelectric manipulator, making possible stable intracellular recordings in awake active animals.

[PubMed - indexed for MEDLINE]
Free full text

LinkOut - more resources

Full Text Sources

Other Literature Sources

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk