Crystallization and preliminary X-ray analysis of the auxin receptor ABP1

E J Woo; J Bauly; J G Chen; J Marshall; H Macdonald; C Lazarus; P Goodenough; M Venis; R Napier; R Pickersgill

doi:10.1107/s0907444900010714

Crystallization and preliminary X-ray analysis of the auxin receptor ABP1

Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1476-8. doi: 10.1107/s0907444900010714.

Authors

E J Woo¹, J Bauly, J G Chen, J Marshall, H Macdonald, C Lazarus, P Goodenough, M Venis, R Napier, R Pickersgill

Affiliation

¹ Queen Mary and Westfield College, Molecular and Cellular Biology, University of London, Mile End Road, London E1 4NS, England.

PMID: 11053858
DOI: 10.1107/s0907444900010714

Abstract

Auxin-binding protein (ABP1) is an important receptor for the plant hormone auxin that is involved with many growth and developmental responses in plants. The maize ABP1 gene has been expressed in insect cells, purified and crystallized. Type II crystals are monoclinic, with two glycosylated homodimers in the asymmetric unit, and diffract to 1.9 A using synchrotron radiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Crystallography, X-Ray
Plant Growth Regulators*
Plant Proteins*
Protein Conformation
Receptors, Cell Surface / chemistry*

Substances

Plant Growth Regulators
Plant Proteins
Receptors, Cell Surface
auxin receptor, plant