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Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1464-5.

Expression, purification and crystallization of human tau-protein kinase I/glycogen synthase kinase-3beta.

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  • 1Mitsubishi Kasei Institute of Life Sciences, 11 Minami-Ooya, Machida, Tokyo 194-8511, Japan.

Abstract

Human tau-protein kinase I (TPK-I; also known as glycogen synthase kinase-3beta, GSK-3beta) is a serine/threonine protein kinase. Full-length TPK-I/GSK-3beta was expressed in Escherichia coli as a fusion protein with a 6xHis tag at the C-terminus and was crystallized using the hanging-drop vapour-diffusion method. Prismatic crystals of dimensions 0.4 x 0.2 x 0.1 mm were obtained using 12-15%(w/v) polyethylene glycol 6000 as a precipitant at 278 K. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 82.9, b = 86.1, c = 178.1 A measured at 100 K, diffract to 2.3 A resolution and seem to contain two enzyme molecules per asymmetric unit.

PMID:
11053853
[PubMed - indexed for MEDLINE]
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