Abstract
The influence of aging on Schwann cell (SC) proliferation, migration and viability was studied in vitro. SCs were cultured in Ham F-10 medium enriched with 20% fetal calf serum (FCS), 40% FCS or collagen I gel plus 20% FCS. The migration of adult mice derived SCs was stimulated with FCS and collagen. With aging, SC migration, multiplication and viability decreased, indicating that ideal culturing conditions should be adjusted.
MeSH terms
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Age Factors
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Aging / physiology
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Animals
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Blood Proteins / pharmacology
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Cell Movement / drug effects
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Cell Movement / physiology*
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Cell Survival / drug effects
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Cell Survival / physiology
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Cells, Cultured
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Collagen / pharmacology
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Culture Media / pharmacology
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Male
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Mice
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Mice, Inbred C57BL
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Schwann Cells / cytology
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Schwann Cells / drug effects
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Schwann Cells / physiology*
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Sciatic Nerve / cytology
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Sciatic Nerve / drug effects
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Sciatic Nerve / physiology*
Substances
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Blood Proteins
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Culture Media
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Collagen