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    Insect Mol Biol. 2000 Oct;9(5):521-9.

    The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae).

    Lessinger AC, Martins Junqueira AC, Lemos TA, Kemper EL, da Silva FR, Vettore AL, Arruda P, Azeredo-Espin AM.

    Source

    Laboratório de Genética Animal,Laboratório Genoma, Instituto de Biologia, Universidade Estadual de Campinas - UNICAMP, Campinas, São Paulo, Brazil.

    Abstract

    The complete sequence of the mitochondrial genome of the screwworm Cochliomyia hominivorax was determined. This genome is 16,022 bp in size and corresponds to a typical Brachycera mtDNA. A Serine start codon for COI and incomplete termination codons for COII, NADH 5 and NADH 4 genes were described. The nucleotide composition of C. hominivorax mtDNA is 77% AT-rich, reflected in the predominance of AT-rich codons in protein-coding genes. Non-optimal codon usage was commonly observed in C. hominivorax mitochondrial genes. Phylogenetic analysis distributed the Acalypterate species as a monophyletic group and assembled the C. hominivorax (Calyptratae) and the Acalyptratae in a typical Brachycera cluster. The identification of diagnostic restriction sites on the sequenced mitochondrial genome and the correlation with previous RFLP analysis are discussed.

    PMID:
    11029671
    [PubMed - indexed for MEDLINE]

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