CEO1, a new protein from Arabidopsis thaliana, protects yeast against oxidative damage

FEBS Lett. 2000 Sep 29;482(1-2):19-24. doi: 10.1016/s0014-5793(00)02016-0.

Abstract

The Saccharomyces cerevisiae strain WYT, deficient in the YAP1 transcription factor, was used in a molecular screen to identify genes from Arabidopsis thaliana that could overcome the oxidative stress-sensitive phenotype of these yeast cells. A cDNA named CEO1 increased the tolerance to oxidative damage caused by tert-butylhydroperoxide of both the Yap1(-) mutant and the wild-type yeast. Additionally, in Yap1(-) yeast, CEO1 also induced cross-tolerance to oxidative damage caused by hydrogen peroxide and diamide. CEO1 was assigned as being part of a small gene family that, until now, is exclusively restricted to plants. In Arabidopsis, CEO1 was produced in all organs, especially in roots and stems. By using the yeast two-hybrid system, proteins that specifically interact with CEO1 in yeast were identified, and putative DNA-binding proteins were consistently recovered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • Escherichia coli
  • Fungal Proteins / genetics
  • Molecular Sequence Data
  • Multigene Family
  • Oxidative Stress
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Roots / metabolism
  • Plant Stems / metabolism
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*

Substances

  • Arabidopsis Proteins
  • CEO1 protein, Arabidopsis
  • DNA-Binding Proteins
  • Fungal Proteins
  • Plant Proteins
  • Protein Isoforms
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • YAP1 protein, S cerevisiae

Associated data

  • GENBANK/AJ251578