Abstract
The Saccharomyces cerevisiae strain WYT, deficient in the YAP1 transcription factor, was used in a molecular screen to identify genes from Arabidopsis thaliana that could overcome the oxidative stress-sensitive phenotype of these yeast cells. A cDNA named CEO1 increased the tolerance to oxidative damage caused by tert-butylhydroperoxide of both the Yap1(-) mutant and the wild-type yeast. Additionally, in Yap1(-) yeast, CEO1 also induced cross-tolerance to oxidative damage caused by hydrogen peroxide and diamide. CEO1 was assigned as being part of a small gene family that, until now, is exclusively restricted to plants. In Arabidopsis, CEO1 was produced in all organs, especially in roots and stems. By using the yeast two-hybrid system, proteins that specifically interact with CEO1 in yeast were identified, and putative DNA-binding proteins were consistently recovered.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics*
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Arabidopsis Proteins*
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Cloning, Molecular
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DNA-Binding Proteins / genetics
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Escherichia coli
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Fungal Proteins / genetics
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Molecular Sequence Data
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Multigene Family
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Oxidative Stress
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plant Roots / metabolism
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Plant Stems / metabolism
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
Substances
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Arabidopsis Proteins
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CEO1 protein, Arabidopsis
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DNA-Binding Proteins
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Fungal Proteins
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Plant Proteins
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Protein Isoforms
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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YAP1 protein, S cerevisiae