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Cell. 2000 Sep 1;102(5):541-4.
Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Department of Medical Chemistry, Graduate School of Medicine, Institute for Virus Research, Kyoto University, Japan.
Induced overexpression of AID in CH12F3-2 B lymphoma cells augmented class switching from IgM to IgA without cytokine stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. AID-/- spleen cells stimulated in vitro with LPS and cytokines failed to undergo class switch recombination although they expressed germline transcripts. Immunization of AID-/- chimera with 4-hydroxy-3-nitrophenylacetyl (NP) chicken gamma-globulin induced neither accumulation of mutations in the NP-specific variable region gene nor class switching. These results suggest that AID may be involved in regulation or catalysis of the DNA modification step of both class switching and somatic hypermutation.
PMID: 11007474 [PubMed - indexed for MEDLINE]
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Cited by over 100 PubMed Central articles
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V-region mutation in vitro, in vivo, and in silico reveal the importance of the enzymatic properties of AID and the sequence environment.
MacCarthy T, Kalis SL, Roa S, Pham P, Goodman MF, Scharff MD, Bergman A.
Proc Natl Acad Sci U S A. 2009 May 26; 106(21):8629-34. Epub 2009 May 14.
[Proc Natl Acad Sci U S A. 2009]
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CD27(-) B-cells produce class switched and somatically hyper-mutated antibodies during chronic HIV-1 infection.
Cagigi A, Du L, Dang LV, Grutzmeier S, Atlas A, Chiodi F, Pan-Hammarström Q, Nilsson A.
PLoS One. 2009; 4(5):e5427. Epub 2009 May 1.
[PLoS One. 2009]
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Impaired antibody response causes persistence of prototypic T cell-contained virus.
Bergthaler A, Flatz L, Verschoor A, Hegazy AN, Holdener M, Fink K, Eschli B, Merkler D, Sommerstein R, Horvath E, et al.
PLoS Biol. 2009 Apr 7; 7(4):e1000080.
[PLoS Biol. 2009]
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