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Acta Crystallogr D Biol Crystallogr. 2000 Oct;56(Pt 10):1215-22.

Macromolecular structure determination by cryo-electron microscopy.

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  • 1Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, England. h.saibil@mail.cryst.bbk.ac.uk

Abstract

Recent advances in transmission electron microscopy (EM) hardware, low-temperature methods and image-processing software have made cryo-EM an important complement to X-ray crystallography and NMR for macromolecular structure determination, particularly of large assemblies. This review provides a summary of the main advances and a survey of the capabilities of this approach.

PMID:
10998617
[PubMed - indexed for MEDLINE]
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