Science. 2000 Sep 15;289(5486):1931-3.

Suppression of mutations in mitochondrial DNA by tRNAs imported from the cytoplasm.

Kolesnikova OA, Entelis NS, Mireau H, Fox TD, Martin RP, Tarassov IA.

Source

FRE 2168 du CNRS, Mécanismes Moléculaires de la Division Cellulaire et du Développement, 21 rue René Descartes, 67084 Strasbourg, France.

Abstract

Mitochondrial import of a cytoplasmic transfer RNA (tRNA) in yeast requires the preprotein import machinery and cytosolic factors. We investigated whether the tRNA import pathway can be used to correct respiratory deficiencies due to mutations in the mitochondrial DNA and whether this system can be transferred into human cells. We show that cytoplasmic tRNAs with altered aminoacylation identity can be specifically targeted to the mitochondria and participate in mitochondrial translation. We also show that human mitochondria, which do not normally import tRNAs, are able to internalize yeast tRNA derivatives in vitro and that this import requires an essential yeast import factor.

PMID:: 10988073; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

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MeSH Terms

Substances

DNA, Mitochondrial

Grant Support

GM29362/GM/NIGMS NIH HHS/United States

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