Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
FEMS Microbiol Lett. 2000 Sep 1;190(1):51-6.

Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes.

Author information

  • 1Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Nagano, Japan.

Abstract

The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphate synthase N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC.

PMID:
10981689
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk