Immunohistochemistry and microwave decalcification of human temporal bones.

Department of Surgery, Division of Otolaryngology, Head and Neck Surgery, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0666, USA. ekeithly@ucsd.edu

Processing of human temporal bones is a long, expensive process and the resulting celloidin sections are difficult to use for immunohistochemistry. We tested the ability of immunohistochemical assays to work in human temporal bones that were decalcified using a microwave oven. Tissue was trimmed to an approximate cube (1.5-2 cm/side) containing only the cochlea and immersed in fresh EDTA with paraformaldehyde every 6 h. This sized block required 190-400 h to decalcify. The decalcified tissue was embedded in paraffin and sectioned. Sections were immunoassayed with anti-cytochrome c oxidase, anti-neurofilament or anti-peripherin. All three antibodies labeled the appropriate structures. This procedure may stimulate advancement in the understanding of human inner ear pathology.

PMID: 10978836 [PubMed - indexed for MEDLINE]