Curr Opin Biotechnol. 2000 Aug;11(4):396-401.

Measuring gene expression by quantitative proteome analysis.

Source

Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA. gygi@u.washington.edu

Abstract

Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the precise quantification and identification of proteins in complex mixtures, that is, quantitative proteomics.

PMID:: 10975460; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Review Advances in quantitative proteomics via stable isotope tagging and mass spectrometry. [Curr Opin Biotechnol. 2003]
Review Advances in quantitative proteomics via stable isotope tagging and mass spectrometry.
Tao WA, Aebersold R. Curr Opin Biotechnol. 2003 Feb; 14(1):110-8.
Complementary analysis of the Mycobacterium tuberculosis proteome by two-dimensional electrophoresis and isotope-coded affinity tag technology. [Mol Cell Proteomics. 2004]
Complementary analysis of the Mycobacterium tuberculosis proteome by two-dimensional electrophoresis and isotope-coded affinity tag technology.
Schmidt F, Donahoe S, Hagens K, Mattow J, Schaible UE, Kaufmann SH, Aebersold R, Jungblut PR. Mol Cell Proteomics. 2004 Jan; 3(1):24-42. Epub 2003 Oct 13.
Review An evaluation of the use of two-dimensional gel electrophoresis in proteomics. [Biomol Eng. 2001]
Review An evaluation of the use of two-dimensional gel electrophoresis in proteomics.
Ong SE, Pandey A. Biomol Eng. 2001 Nov; 18(5):195-205.
Review Current two-dimensional electrophoresis technology for proteomics. [Proteomics. 2004]
Review Current two-dimensional electrophoresis technology for proteomics.
Görg A, Weiss W, Dunn MJ. Proteomics. 2004 Dec; 4(12):3665-85.
Review Mass spectrometry-based quantitative proteomic profiling. [Brief Funct Genomic Proteomic....]
Review Mass spectrometry-based quantitative proteomic profiling.
Yan W, Chen SS. Brief Funct Genomic Proteomic. 2005 May; 4(1):27-38.

See reviews... See all...

Cited by 11 PubMed Central articles

Proteomics: a biotechnology tool for crop improvement. [Front Plant Sci. 2013]
Proteomics: a biotechnology tool for crop improvement.
Eldakak M, Milad SI, Nawar AI, Rohila JS. Front Plant Sci. 2013; 4:35. Epub 2013 Feb 28.
Janus-like opposing roles of CD47 in autoimmune brain inflammation in humans and mice. [J Exp Med. 2012]
Janus-like opposing roles of CD47 in autoimmune brain inflammation in humans and mice.
Han MH, Lundgren DH, Jaiswal S, Chao M, Graham KL, Garris CS, Axtell RC, Ho PP, Lock CB, Woodard JI, et al. J Exp Med. 2012 Jul 2; 209(7):1325-34. Epub 2012 Jun 25.
Review Exploring mixed microbial community functioning: recent advances in metaproteomics. [FEMS Microbiol Ecol. 2012]
Review Exploring mixed microbial community functioning: recent advances in metaproteomics.
Siggins A, Gunnigle E, Abram F. FEMS Microbiol Ecol. 2012 May; 80(2):265-80. Epub 2012 Jan 16.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Measuring gene expression by quantitative proteome analysis.
Measuring gene expression by quantitative proteome analysis.
Curr Opin Biotechnol. 2000 Aug ;11(4):396-401.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: