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Infect Control Hosp Epidemiol. 2000 Aug;21(8):525-7.

Use of spoligotype analysis to detect laboratory cross-contamination.

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  • 1New York City Department of Health, Tuberculosis Control Program, New York 10007, USA.


Spoligotype analysis identified false-positive isolates of Mycobacterium tuberculosis caused by laboratory cross-contamination. Spoligotyping is faster, is less expensive than DNA fingerprinting, and can be used with a variety of media. Patients were reevaluated and had medications discontinued as a result of this investigation. Months of unnecessary patient follow-up and treatment were avoided.

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