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1: Biol Signals Recept. 2000 Sep-Oct;9(5):240-7.Click here to read Links

Immunoglobulin G1 Fc fragment-tagged human opioid receptor-like receptor retains the ability to inhibit cAMP accumulation.

Yung LY, Tsim KW, Pei G, Wong YH.

Department of Biology and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.

The human opioid receptor-like (ORL(1)) receptor was tagged with the immunoglobulin G1 Fc fragment at the carboxy-terminus and expressed in human embryonic kidney 293 cells. Expression of the ORL(1)-Fc receptor was confirmed by immunohistochemical staining. The fusion protein was enriched by affinity chromatography and then verified by immunodetection. The function of the ORL(1)-Fc receptor was determined by examining nociceptin/OFQ-induced inhibition of cAMP accumulation. The ORL(1)-Fc receptor inhibited the forskolin-stimulated cAMP accumulation. The inhibitory response was selectively induced by nociceptin/OFQ in a dose-dependent and pertussis toxin-sensitive manner. Our results indicate that the carboxy-terminal Fc-tagged ORL(1) receptor retained the ability to interact with G(i) proteins to inhibit adenylyl cyclase. Copyright 2000 S. Karger AG, Basel.

PMID: 10965058 [PubMed - indexed for MEDLINE]