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    Biochem Biophys Res Commun. 2000 Aug 28;275(2):623-30.

    Genomic structure and in vivo expression of the human organic anion transporter 1 (hOAT1) gene.

    Bahn A, Prawitt D, Buttler D, Reid G, Enklaar T, Wolff NA, Ebbinghaus C, Hillemann A, Schulten HJ, Gunawan B, Füzesi L, Zabel B, Burckhardt G.

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    Zentrum für Physiologie und Pathophysiologie, Humboldtallee 23, Göttingen, 37073, Germany. abahn@veg-physiol.med.uni-goettingen.de

    Abstract

    The human organic anion transporter 1 (hOAT1) plays a key role in the secretion of an array of potentially toxic organic anions including many clinically important drugs. Here we report on the genomic cloning of hOAT1. A human genomic library was used for screening of a PAC (P1 artificial chromosome) clone applying PCR techniques. Sequencing of several restriction subclones and of a PCR-generated clone revealed that the hOAT1 gene spans 8.2 kb and is composed of 10 exons divided by 9 introns. RT-PCR studies in a human kidney specimen led to the detection of two new splice variants, hOAT1-3 and hOAT1-4, showing a 132-bp in-frame deletion. Using fluorescence in situ hybridization (FISH) we mapped the hOAT1 gene as a single signal to chromosome 11q13.1-q13.2. Additionally, 600 bp of the 5' flanking region was analyzed, illustrating the probable transcription start site at nt -280, a NF-kappaB-site at nt -397 and several putative transcription factor binding sites.

    Copyright 2000 Academic Press.

    PMID:
    10964714
    [PubMed - indexed for MEDLINE]

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