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Plasmid. 2000 Sep;44(2):201-7.

A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria.

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  • 1Plasticité et Expression des Génomes Microbiens, CNRS EP2029, CEA LRC N degrees 12, Université Joseph Fourier, 460 rue de la Piscine, Grenoble Cedex 9, F-38041, France.


Plasmid pGBG1 was constructed to isolate mobile genetic elements in a wide variety of gram-negative bacteria. The mutation target, carried on a broad-host-range vector, allows positive selection for tetracycline resistance. In tests using several gram-negative bacteria we could detect transposition events of either insertion sequences or transposons. A new insertion sequence (IS) element was identified in Ralstonia eutropha.

Copyright 2000 Academic Press.

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