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J Exp Zool. 2000 Sep 1;287(4):285-93.

Introduction of a foreign gene into medakafish using the particle gun method.

Author information

  • 1Genome Research Group, National Institute of Radiological Sciences, Chiba 263-8555, Japan. m_yam@nirs.go.jp

Abstract

We developed a procedure to introduce a foreign gene into fertilized eggs of medakafish (Oryzias latipes) using the particle gun method, which is one of the easiest and most reliable techniques for gene transfer. A plasmid construct with the green fluorescence protein (GFP) gene driven by the madakafish beta-actin gene promoter was successfully introduced into eggs, and the expression of GFP was observed in 20% of the primary transfectant (chimera) fish. In addition, germ line transmission of GFP was observed in 13% of the GFP-positive primary transfectant fish. The new application described here should enable us to investigate gene expression using the fish model on a routine basis without high technical sophistication. J. Exp. Zool. 287:285-293, 2000.

Copyright 2000 Wiley-Liss, Inc.

PMID:
10951388
[PubMed - indexed for MEDLINE]
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