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Plant Cell Physiol. 2000 Jun;41(6):811-6.

A rapid and efficient system of Agrobacterium infection-mediated transient gene expression in rice Oc cells and its application for analysis of the expression and antisense suppression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene.

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  • 1Laboratory of Bioactive Natural Product Chemistry, Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Japan. hyang@agr.nagoya-u.ac.jp

Abstract

A rapid and efficient system for Agrobacterium infection-mediated transient gene expression in rice has been developed. Using this system, transient expression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene was analyzed. The results suggest that the Agrobacterium infection-mediated transient gene expression system is as efficient in rice Oc cells as in tobacco BY-2 cells and might be useful for rapid analysis not only of foreign gene expression, but also of antisense gene suppression.

PMID:
10945352
[PubMed - indexed for MEDLINE]
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