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J Bacteriol. 2000 Sep;182(17):5025-8.

Reduction of GC --> TA transversion mutation by overexpression of MutS in Escherichia coli K-12.

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  • 1Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA.


Overexpression of the MutS repair protein significantly decreased the rate of lacZ GC --> TA transversion mutation in stationary-phase and exponentially growing bacteria and in mutY and mutM mutants, which accumulate mismatches between 8-oxoguanine (8-oxoG) and adenine residues in DNA. Conversely, GC --> TA transversion increased in mutL or mutS mutants in stationary phase. In contrast, overexpression of MutS did not appreciably reduce lacZ AT --> CG transversion mutation in a mutT mutant. These results suggest that MutS-dependent repair can correct 8-oxoG:A mismatches in Escherichia coli cells but may not be able to compete with mutation fixation by MutY in mutT mutants.

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