Blastocystis hominis: neutral red supravital stain...[Parasitol Res. 2000]

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1: Parasitol Res. 2000 Jul;86(7):573-81. Links

Blastocystis hominis: neutral red supravital staining and its application to in vitro drug sensitivity testing.

Vdovenko AA, Williams JE.

University of Hawaii, Dept. of Pathology, Honolulu, 96822, USA. Vdovenko@aol.com

A simple in vitro drug sensitivity testing system for Blastocystis hominis clinical isolates was developed. Application of supravital staining by neutral red allowed quantitative viability assessment. Four xenic cultures, isolated from human sources, were grown in modified monophasic Robinson's medium and tested for sensitivities to nine available drugs. Assessment was done using the cell-count method from air-dried preparations after supravital staining with neutral red. Also, the light absorbence method was evaluated. Trimethoprim, metronidazole, quinacrine, tetracycline, paromomycin, and two new antiprotozoal drugs, nitazoxanide and deacetyl-nitazoxanide, showed cytostatic or cytocidal effects on all or some Blastocystis isolates. Chloroquine and sulphamethoxazole did not demonstrate any effect at the concentrations studied.

PMID: 10935909 [PubMed - indexed for MEDLINE]

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Tetracycline (Helidac Therapy® as a combination product containing Tetracycline, Metronidazole, and Bismuth subsalicylate, Sumycin® , Sumycin® Syrup)
Tetracycline, is used to treat bacterial infections, including pneumonia and other respiratory tract infections; acne; infections of skin, genital and urinary systems; and the infection that causes stomach ulcers (Helico...
Trimethoprim (Proloprim® )
Trimethoprim eliminates bacteria that cause urinary tract infections. It is used in combination with other drugs to treat certain types of pneumonia. It also is used to treat 'travelers' diarrhea.' Antibiotics will not w...
Nitazoxanide (Alinia® )
Nitazoxanide is used to treat diarrhea in children and adults caused by the protozoaCryptosporidium orGiardia. Protozoa are suspected as the cause when diarrhea lasts more than 7 days. Nitazoxanide is in a class of medic...
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