FEBS Lett. 2000 Aug 4;478(3):241-5.

ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan.

Kuno K, Okada Y, Kawashima H, Nakamura H, Miyasaka M, Ohno H, Matsushima K.

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Department of Molecular Membrane Biology, Cancer Research Institute, Kanazawa University, Ishikawa, Japan. kkuno@kenroku.kanazawa-u.ac.jp

Abstract

A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu(1871)-Leu(1872) bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo.

PMID:: 10930576; [PubMed - indexed for MEDLINE]

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ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan.
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FEBS Lett. 2000 Aug 4 ;478(3):241-5.

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