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J Clin Microbiol. 2000 Aug;38(8):3092-5.

Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci.

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  • 1Section of Infection Control, Okayama University Hospital, Okayama 700-8558, Japan. kariyama@med.okayama-u.ac.jp

Abstract

Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

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PMID:
10921985
[PubMed - indexed for MEDLINE]
PMCID:
PMC87194
Free PMC Article
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