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J Am Vet Med Assoc. 2000 Jul 15;217(2):220-5.

Association of disease with isolation and virulence of Rhodococcus equi from farm soil and foals with pneumonia.

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  • 1Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843, USA.

Abstract

OBJECTIVE:

To determine whether isolation and virulence of Rhodococcus equi from soil and infected foals are associated with clinical disease.

DESIGN:

Cross-sectional and case-control study.

SAMPLE POPULATION:

R equi isolates from 50 foals with pneumonia and soil samples from 33 farms with and 33 farms without a history of R equi infection (affected and control, respectively).

PROCEDURE:

R equi was selectively isolated from soil samples. Soil and clinical isolates were evaluated for virulence-associated protein antigen plasmids (VapA-P) and resistance to the beta-lactam antibiotics penicillin G and cephalothin. Microbiologic cultures and VapA-P assays were performed at 2 independent laboratories.

RESULTS:

VapA-P was detected in 49 of 50 (98%) clinical isolates; there was complete agreement between laboratories. Rhodococcus equi was isolated from soil on 28 of 33 (84.8%) affected farms and 24 of 33 (72.7%) control farms, but there was poor agreement between laboratories. Virulence-associated protein antigen plasmids were detected on 14 of 66 (21.2%) farms by either laboratory, but results agreed for only 1 of the 14 VapA-P-positive farms. We did not detect significant associations between disease status and isolation of R equi from soil, detection of VapA-P in soil isolates, or resistance of soil isolates to beta-lactam antibiotics. No association between beta-lactam antibiotic resistance and presence of VapA-P was detected.

CONCLUSIONS AND CLINICAL RELEVANCE:

On the basis of soil microbiologic culture and VapA-P assay results, it is not possible to determine whether foals on a given farm are at increased risk of developing disease caused by R equi.

PMID:
10909463
[PubMed - indexed for MEDLINE]
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