Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Yeast. 2000 Jun 30;17(2):88-94.

Yeast two-hybrid systems and protein interaction mapping projects for yeast and worm.

Author information

  • 1Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Abstract

The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as 'functional genomics'. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein-protein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential protein-protein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans.

Copyright 2000 John Wiley & Sons, Ltd.

PMID:
10900455
[PubMed - indexed for MEDLINE]
PMCID:
PMC2448329
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for John Wiley & Sons, Inc. Icon for PubMed Central
    Loading ...
    Write to the Help Desk