Apoptosis induced by the flavonoid from lemon fruit (Citrus limon BURM. f.) and its metabolites in HL-60 cells

S Ogata; Y Miyake; K Yamamoto; K Okumura; H Taguchi

doi:10.1271/bbb.64.1075

Apoptosis induced by the flavonoid from lemon fruit (Citrus limon BURM. f.) and its metabolites in HL-60 cells

Biosci Biotechnol Biochem. 2000 May;64(5):1075-8. doi: 10.1271/bbb.64.1075.

Authors

S Ogata¹, Y Miyake, K Yamamoto, K Okumura, H Taguchi

Affiliation

¹ Department of Life Science, Faculty of Bioresources, Mie University, Tsu, Japan.

PMID: 10879486
DOI: 10.1271/bbb.64.1075

Abstract

The flavonoid from lemon fruit (Citrus limon BURM. f.) and its metabolites, particularly eriodictyol, 3,4-dihydroxyhydrocinnamic acid, and phloroglucinol had the function of DNA fragmentation in HL-60 cells when analyzed by flow cytometry. An apoptotic DNA ladder and chromatin condensation were observed in HL-60 cells when treated with these compounds. The caspase inhibitor prevented DNA fragmentation. These compounds are anticipated to be useful for medical purposes.

MeSH terms

Apoptosis / drug effects
Apoptosis / physiology*
Caffeic Acids / pharmacology
Caspase Inhibitors
Cell Size
Citrus / chemistry*
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Electrophoresis, Agar Gel
Flavanones*
Flavonoids / chemistry
Flavonoids / metabolism*
Flavonoids / pharmacology*
HL-60 Cells
Hesperidin / analogs & derivatives*
Hesperidin / pharmacology
Humans
Phloroglucinol / pharmacology

Substances

Caffeic Acids
Caspase Inhibitors
Flavanones
Flavonoids
eriocitrin
Phloroglucinol
Hesperidin
eriodictyol
caffeic acid