Science. 2000 Jun 16;288(5473):1973-5.

Reversal of fortune for Drosophila geneticists?

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Department of Genetics, University of Wisconsin, Madison, WI 53706, USA. wrengels@facstaff.wisc.edu

Abstract

Although Drosophila is a wonderful model organism, there is one molecular arena where it lags far behind its yeast and mouse model counterparts. Reverse genetics, whereby a piece of DNA is integrated into a target gene such that the gene is disrupted or replaced, is not easy in Drosophila. As Engels explains in his provocative Perspective, this may be set to change with the description of a new method for reverse genetics in Drosophila (Rong and Golic). This new technique should ensure that Drosophila remains the darling of geneticists for many years to come.

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Gene targeting by homologous recombination in Drosophila. [Science. 2000]
Gene targeting by homologous recombination in Drosophila.Rong YS, Golic KG. Science. 2000 Jun 16; 288(5473):2013-8.

PMID:: 10877715; [PubMed - indexed for MEDLINE]

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DNA Transposable Elements

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Gene targeting by homologous recombination in Drosophila. [Science. 2000]
Gene targeting by homologous recombination in Drosophila.
Rong YS, Golic KG. Science. 2000 Jun 16; 288(5473):2013-8.
Gene targeting of a plasmid-borne sequence to a double-strand DNA break in Drosophila melanogaster. [Mol Cell Biol. 1996]
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Cited by 5 PubMed Central articles

Knockout targeting of the Drosophila nap1 gene and examination of DNA repair tracts in the recombination products. [Genetics. 2003]
Knockout targeting of the Drosophila nap1 gene and examination of DNA repair tracts in the recombination products.
Lankenau S, Barnickel T, Marhold J, Lyko F, Mechler BM, Lankenau DH. Genetics. 2003 Feb; 163(2):611-23.
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. [Genetics. 2002]
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases.
Bibikova M, Golic M, Golic KG, Carroll D. Genetics. 2002 Jul; 161(3):1169-75.
Targeted mutagenesis by homologous recombination in D. melanogaster. [Genes Dev. 2002]
Targeted mutagenesis by homologous recombination in D. melanogaster.
Rong YS, Titen SW, Xie HB, Golic MM, Bastiani M, Bandyopadhyay P, Olivera BM, Brodsky M, Rubin GM, Golic KG. Genes Dev. 2002 Jun 15; 16(12):1568-81.

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