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Human blood group 0 cells were converted into B-active cells by incubation with uridine diphosphate D-galactose and unfractionated serum from bull frog (Rana catesbeiana). Under these conditions, "Bombay" (Oh) type red cells were not converted, and group 0 foetal cells possessing a few H antigenic sites were only weakly converted into B-active cells. In addition, the agglutination titer of the converted cells with anti-H serum decreased. These results therefore indicate that the conversion depends upon the presence of H-substance on the red cells. It was concluded from the results of this investigation that the blood group B specific alpha-galactosyltransferase was present in the serum of Rana catesbeiana as well as in the human group B serum.
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