Specific antibody for chum salmon somatolactin (SL) was used for immunocytochemical investigation of SL cell activity of Mugil cephalus during the gonadal cycle in both natural habitat and captivity. The SL-immunoreactive cells showed strong and specific immunoreactivity to antichum salmon SL. The number of SL-immunoreactive cells increased, as did the secretory and synthetic activity during sexual maturation and spawning in the natural habitat. The SL cells were rather small and moderately immunoreactive in immature fish; they were enlarged, their numbers increased, and they frequently showed more SL immunoreactivity during gonadal development. In addition, during late stages of maturation, small cell size with more or less SL immunoreactive cells were noted, indicating an active release of SL granules. Prespawning females tended to have more enlarged SL cells with stronger immunoreactivity than equivalent males. The SL cells showed an increase in the secretory activity during spawning as indicated by small size and weak immunoreactivity. The SL cells of M. cephalus reared in captivity showed high activity. This may be due to the low concentration of calcium in fresh water. The gradual stimulation of SL synthesis and release during sexual maturation and spawning of M. cephalus suggest that SL may be involved in the control of some steps of reproductive processes, such as steroidogenesis, calcium metabolism, and energy mobilization.