p53 colocalizes with expanded httex1p in inclusions in mammalian cell culture and interacts with httex1p in vitro and in cell culture. (A) Western blot of solubilized proteins purified from aggregates generated in HEK293 cells expressing 103Q-GFP. Results were identical to aggregates purified from 103QP-GFP (data not shown). WC denotes the whole-cell fraction from transfected cells and AGG the aggregate preparation. Equivalent ratios of WC:AGG were run on 8% SDS gels and Western blotted. Antisera were used to examine relative levels of 103Q-GFP htt, p53, CBP, mSin3a, mdm2, AR, NF-κB p65, and RXRα in the aggregate preparation. No immunoreactive htt protein was present in samples corresponding to the aggregate fraction isolated from 293 cells expressing 25Q-GFP (data not shown). (B) Binding of [³⁵S]methionine-labeled p53(1–393) and p53(1–347) to GST-htt fusion proteins. Equivalent amounts of [³⁵S]methionine-labeled p53(1–393) and p53(1–347) were mixed with GST-20QP, GST-51QP, GST, and GST-103Q attached to glutathione-agarose beads, incubated, and washed. The labeled proteins bound to the beads were analyzed by SDS-PAGE. Each experiment was done in triplicate. Percent binding ± SE was calculated by phosophorimager analysis and is listed below each lane. Ten percent of the input of the labeled proteins was also analyzed as shown. Slight alteration in the pattern of p53 migration in the GST-51QP lane as compared with GST-20QP lane is because of large amounts GST-51QP comigrating with labeled p53. (C) Coprecipitation of p53 with httex1p in HEK293 cells. HEK293 cells were transiently transfected with plasmids encoding p53 and either GST, GST-20QP, GST-83QP, or GST-103Q. Cells were broken and lysates were incubated with glutathione-agarose beads. After extensive washing, beads were loaded directly on an 8% SDS gel. Western blot analysis using monoclonal anti-p53 antibody is shown.

CBP and mSin3a bind to httex1p in vitro and CBP localizes to nuclear inclusions in R6/2 mouse brains. (Top) Binding of [³⁵S]methionine-labeled CBP or mSin3a to GST-htt fusion proteins. Equivalent amounts of [³⁵S]methionine-labeled CBP (I) or mSin3a (II) were mixed with GST, GST-20QP, GST-51QP, GST-103Q, or GST-p53 attached to glutathione-agarose beads, washed, and the labeled proteins bound to the beads were analyzed by SDS-PAGE. Each experiment was done in triplicate. Percent binding ± SE was calculated by phosphorimager analysis and are listed below each lane. Ten percent of the input of the labeled proteins was also analyzed as shown. (A–D) Localization of CBP to neuronal intranuclear inclusions in R6/2 mice. Striatal sections from R6/2 (A–C) and littermate controls (D) were immunolabeled with anti-htt (A), -ubiquitin (B), and -CBP (C and D) antibodies. Nuclei were counterstained with methyl green. Nuclear inclusions are indicated by arrows. Scale bar, 15 μm.

Expanded httex1p represses transcription. (Upper) SAOS-2 cells were transfected with vector alone (control) or plasmids encoding 25QP-GFP, 103QP-GFP, 25Q-myc, or 103Q-myc proteins and plasmids encoding WAF1-luciferase and p53. (Lower) SAOS-2 cells were transfected with vector alone (control) or plasmids encoding 20QP, 93QP, 25Q-myc, or 103-myc proteins and the MDR1-luciferase reporter plasmid. A p53-encoding plasmid was also transfected as indicated. Each experiment was done in triplicate. Luciferase units per milligram of protein were calculated and are demonstrated graphically as percent of vector control values.