Mammary epithelial cells contact a specialized extracellular matrix in vivo known as the basement membrane. Interactions with extracellular matrix are mediated through integrins. These cell surface receptors are involved with the formation of adhesion complexes, which link the extracellular matrix with the actin-based cytoskeleton, and are also associated with components of growth factor signaling pathways. Differentiation of breast epithelia into lactational cells requires appropriate hormones and integrin-mediated interactions with basement membrane. Integrins may regulate the ability of lactogenic hormones to trigger their intracellular signaling pathways.