Org Lett. 2000 May 4;2(9):1307-10.

Mechanistic studies of the tRNA-modifying enzyme QueA: a chemical imperative for the use of AdoMet as a "ribosyl" donor.

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Department of Chemistry, Portland State University, Oregon 97201, USA.

Abstract

[formula: see text] The enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) catalyzes the penultimate step in the biosynthesis of the tRNA nucleoside queuosine, a unique ribosyl transfer from the cofactor S-adenosylmethionine (AdoMet) to a modified-tRNA precursor. The use of AdoMet in this way is fundamentally new to the chemistry of this important biological cofactor. We report here the first mechanistic studies of this remarkable enzyme, and we propose a chemical mechanism for the reaction consistent with our experimental observations.

PMID:: 10810734; [PubMed - indexed for MEDLINE]

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Kinetic mechanism of the tRNA-modifying enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA). [Biochemistry. 2003]
Kinetic mechanism of the tRNA-modifying enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA).
Van Lanen SG, Iwata-Reuyl D. Biochemistry. 2003 May 13; 42(18):5312-20.
Transfer and isomerization of the ribose moiety of AdoMet during the biosynthesis of queuosine tRNAs, a new unique reaction catalyzed by the QueA protein from Escherichia coli. [Biochimie. 1994]
Transfer and isomerization of the ribose moiety of AdoMet during the biosynthesis of queuosine tRNAs, a new unique reaction catalyzed by the QueA protein from Escherichia coli.
Slany RK, Bösl M, Kersten H. Biochimie. 1994; 76(5):389-93.
A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine. [Biochemistry. 1993]
A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine.
Slany RK, Bösl M, Crain PF, Kersten H. Biochemistry. 1993 Aug 3; 32(30):7811-7.
Review Biosynthesis of the 7-deazaguanosine hypermodified nucleosides of transfer RNA. [Bioorg Chem. 2003]
Review Biosynthesis of the 7-deazaguanosine hypermodified nucleosides of transfer RNA.
Iwata-Reuyl D. Bioorg Chem. 2003 Feb; 31(1):24-43.
Review [tRNA guanine transglycosylase and biosynthesis of Q nucleoside present in tRNA as a minor component (author's transl)]. [Seikagaku. 1982]
Review [tRNA guanine transglycosylase and biosynthesis of Q nucleoside present in tRNA as a minor component (author's transl)].
Okada N. Seikagaku. 1982; 54(2):79-94.

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Cited by 2 PubMed Central articles

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