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Plant Physiol. 2000 May;123(1):287-96.

Metabolism of methanol in plant cells. Carbon-13 nuclear magnetic resonance studies.

Author information

  • 1Laboratoire de Résonance Magnétique en Biologie Métabolique, Département de Biologie Moléculaire et Structurale, CEA-38054, Grenoble cedex 9, France.

Abstract

Using (13)C-NMR, we demonstrate that [(13)C]methanol readily entered sycamore (Acer pseudoplatanus L.) cells to be slowly metabolized to [3-(13)C]serine, [(13)CH(3)]methionine, and [(13)CH(3)]phosphatidylcholine. We conclude that the assimilation of [(13)C]methanol occurs through the formation of (13)CH(3)H(4)Pte-glutamate (Glu)(n) and S-adenosyl-methionine, because feeding plant cells with [3-(13)CH(3)]serine, the direct precursor of (13)CH(2)H(4)Pte-Glu(n), can perfectly mimic [(13)CH(3)]methanol for folate-mediated single-carbon metabolism. On the other hand, the metabolism of [(13)C]methanol in plant cells revealed assimilation of label into a new cellular product that was identified as [(13)CH(3)]methyl-beta-D-glucopyranoside. The de novo synthesis of methyl-beta-D-glucopyranoside induced by methanol did not require the formation of (13)CH(3)H(4)Pte-Glu(n) and was very likely catalyzed by a "transglycosylation" process.

PMID:
10806245
[PubMed - indexed for MEDLINE]
PMCID:
PMC59002
Free PMC Article

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