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Biochem Biophys Res Commun. 2000 May 10;271(2):334-6.

Cloning and expression of beta,beta-carotene 15,15'-dioxygenase.

Author information

  • 1Vitamins & Fine Chemicals Division, F. Hoffmann-LaRoche Ltd., Basel, 4070, Switzerland. adrian.wyss@roche.com

Abstract

beta,beta-Carotene 15,15'-dioxygenase cleaves beta-carotene into two molecules of retinal and is therefore the key enzyme in beta-carotene metabolism to vitamin A. In the present study, it was possible to enrich the chicken beta,beta-carotene 15,15'-dioxygenase to such an extent that partial amino acid sequence information could be obtained to design degenerate oligonucleotides. With RT-PCR a cDNA fragment could be obtained and used subsequently in a radioactive screening of a chicken duodenal expression library. We cloned the first eukaryotic beta,beta-carotene 15,15'-dioxygenase which symmetrically cleaves beta-carotene at the 15,15'-double bond.

Copyright 2000 Academic Press.

PMID:
10799297
[PubMed - indexed for MEDLINE]
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