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Anal Biochem. 2000 May 1;280(2):315-9.

Assay for identification of inhibitors for bacterial MraY translocase or MurG transferase.

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  • 1Department of Biological Research, IRL, Inc., Cranbury, New Jersey, 08512, USA.


Bacterial peptidoglycan synthesis is a well-characterized system for targeting new antimicrobial drugs. Formation of the peptidoglycan precursors Lipid I and Lipid II is catalyzed by the gene products of mraY and murG, which are involved in the first and second steps of the lipid cycle reactions, respectively. Here we describe the development of an assay specific for identifying inhibitors of MraY or MurG, based on the detection of radiolabeled [(14)C]GlcNAc incorporated into Lipid II. Assay specificity is achieved with the biotin tagging of the Lipid I precursor UDP-MurNAc-pentapeptide. This allows for the separation and identification of lipid products produced by the enzymatic activity of the MraY and MurG proteins, and thus identification of specific inhibitors.

Copyright 2000 Academic Press.

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