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Pflugers Arch. 2000 Apr;439(6):714-22.

Cloning, localisation and functional expression of the human orthologue of the TREK-1 potassium channel.

Author information

  • 1Neuroscience Research, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK. Helen_J_Meadows@sbphrd.com

Abstract

We have cloned human TREK-1, one of the newly emerging mammalian family of 2-P domain potassium channels. The channel has 411 amino acids with a 41-amino-acid extension at the C-terminus when compared with the cloned mouse TREK-1 channel. Expression of hTREK-1 produced a substantial hyperpolarising shift in resting membrane potential accompanied by the induction of large, outwardly rectifying, non-inactivating currents which were potassium selective. Pharmacologically, hTREK-1-mediated currents were only blocked to a limited extent by classic potassium channel blockers or open channel pore blockers known to potently inhibit other channels. The channel was reversibly potentiated by arachidonic acid. CNS distribution of hTREK-1 is widespread with higher levels being observed in caudate, putamen, amygdala, thalamus and spinal cord. Only low levels of expression were seen in the majority of peripheral regions. Thus, hTREK-1, although functionally and pharmacologically similar to mouse TREK-1, appears to have a more CNS-specific distribution.

PMID:
10784345
[PubMed - indexed for MEDLINE]
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