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J Magn Reson. 2000 May;144(1):162-7.

Imaging membrane protein helical wheels.

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  • 1National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310, USA.


Resonance patterns have been observed in 2D solid-state NMR spectra of the transmembrane segment of M2 protein from Influenza A virus in oriented samples reflecting the helical wheel of this alpha-helix. The center of this pattern uniquely defines the helical tilt with respect to the bilayer normal without a need for resonance assignments. The distribution of resonances from amino acid specific labels around the "PISA wheel" defines the rotational orientation of the helix and yields preliminary site-specific assignments. With assignments high-resolution structural detail, such as differences in tilt and rotational orientation along the helical axis leading to an assessment of helical coiling, can be obtained.

Copyright 2000 Academic Press.

[PubMed - indexed for MEDLINE]
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