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Science. 2000 Apr 14;288(5464):316-8.

Translating biomolecular recognition into nanomechanics.

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  • 1IBM Research, Zurich Research Laboratory, Säumerstrasse 4, CH-8803 Rüschlikon, Switzerland.

Abstract

We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.

PMID:
10764640
[PubMed - indexed for MEDLINE]
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