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Curr Opin Biotechnol. 2000 Apr;11(2):157-61.

Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project.

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  • 11 Research Link, Institute of Molecular Agrobiology, The National University of Singapore, 117604, Singapore.


The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet kept up to this pace. Recent progress in large-scale insertional mutagenesis opens new possibilities for functional genomics in Arabidopsis. The number of T-DNA and transposon insertion lines from different laboratories will soon represent insertions into most Arabidopsis genes. Vast resources of gene knockouts are becoming available that can be subjected to different types of reverse genetics screens to deduce the functions of the sequenced genes.

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