Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Science. 2000 Apr 7;288(5463):136-40.

Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons.

Author information

  • 1Genome Sciences Department, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

Long-range regulatory elements are difficult to discover experimentally; however, they tend to be conserved among mammals, suggesting that cross-species sequence comparisons should identify them. To search for regulatory sequences, we examined about 1 megabase of orthologous human and mouse sequences for conserved noncoding elements with greater than or equal to 70% identity over at least 100 base pairs. Ninety noncoding sequences meeting these criteria were discovered, and the analysis of 15 of these elements found that about 70% were conserved across mammals. Characterization of the largest element in yeast artificial chromosome transgenic mice revealed it to be a coordinate regulator of three genes, interleukin-4, interleukin-13, and interleukin-5, spread over 120 kilobases.

Comment in

PMID:
10753117
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk