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EMBO J. 2000 Apr 3;19(7):1494-504.

GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28.

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  • 1Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel.

Abstract

Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.

PMID:
10747018
[PubMed - indexed for MEDLINE]
PMCID:
PMC310219
Free PMC Article

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