Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Trends Biotechnol. 2000 Apr;18(4):147-51.

Nanopores and nucleic acids: prospects for ultrarapid sequencing.

Author information

  • 1Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA. deamer@hydrogen.ucsc.edu

Abstract

DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers. If single-nucleotide resolution can be achieved, it is possible that nucleic acid sequences can be determined at rates exceeding a thousand bases per second.

PMID:
10740260
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk